| Emmanuelle Vaugier | ... | Detective Jessica Angell (25 episodes) |
| Claire Forlani | ... | Dr. Peyton Driscoll (11 episodes) |
| Sonya Walger | ... | Jane Parsons (10 episodes) |
| Danielle McKee | ... | Lab Tech (7 episodes) |
| Kyle Gallner | ... | Reed Garrett (7 episodes) |
| Mykelti Williamson | ... | Chief Brigham Sinclair (7 episodes) |
| Kacey Taylor | ... | Lab Tech (7 episodes) |
| Carmen Argenziano | ... | Insp. Stanton Gerrard (6 episodes) |
| J. Grant Albrecht | ... | Dr. Leonard Giles (5 episodes) |
| Vanessa Esperanza | ... | Monica (5 episodes) |
| Chad Lindberg | ... | Chad Willingham (5 episodes) |
| Edward Furlong | ... | Shane Casey (5 episodes) |
| Nicholas Pratley | ... | Ryan Mallone (4 episodes) |
| Kelly Hu | ... | Detective Kaile Maka (4 episodes) |
| Bess Wohl | ... | Audrey Davis (4 episodes) |
| Ron Yuan | ... | Dr. Evan Zao (4 episodes) |
| Ed Quinn | ... | Frankie Mala (4 episodes) |
| Jonah Lotan | ... | Dr. Marty Pino (4 episodes) |
| Kerr Smith | ... | Drew Bedford (4 episodes) |
| Jacqueline Pinol | ... | Rikki Sandoval (4 episodes) |
| Nelly | ... | Terrence Davis (4 episodes) |
| Paul Papadakis | ... | George Kolovos (4 episodes) |
| Denice Sealy | ... | Tech (Raquel Trinidad) (3 episodes) |
| Kanin Howell | ... | Lenny Starks (3 episodes) |
| David Julian Hirsh | ... | Zack Shannon (3 episodes) |
| John Dove | ... | Det. John Scagnetti (3 episodes) |
| Joseph Lawrence | ... | Clay Dobson (3 episodes) |
| Jessalyn Gilsig | ... | Jordan Gates (3 episodes) |
| Kathleen Munroe | ... | Samantha Flack (3 episodes) |
| Casey LaBow | ... | Ella McBride (3 episodes) |
| Julia Ormond | ... | Deputy Inspector Gillian Whitford (3 episodes) |
| Craig T. Nelson | ... | Robert Dunbrook (3 episodes) |
| Tony Amendola | ... | Professor Papakota (3 episodes) |
| Sarah Carter | ... | Haylen Becall (3 episodes) |
| Josie Davis | ... | Calliope Eckhart (3 episodes) |
| Skeet Ulrich | ... | Hollis Eckhart (3 episodes) |
| Olivia Summers | ... | Carole Hillcroft (3 episodes) |
| Kevin Navayne | ... | Detective Ray Jackson (3 episodes) |
| Mädchen Amick | ... | Aubrey Hunter (3 episodes) |
| John Larroquette | ... | Chief Ted Carver (3 episodes) |
